Unfilterable Beer Haze Part II: Identifying Suspect Cell Wall Proteins

نویسندگان

چکیده

The use of various diagnostic techniques has been previously utilized in the assessment a commercially available India Pale Ale with cases sporadically occurring unfilterable haze. results from Part 1 suggested that β-glucans and proteins were cause haze it was postulated cell wall mannoproteins may also be culprit beer In this follow-up study, high low samples precipitated assessed using SDS-PAGE. Polyphenol interferences observed on SDS-PAGE indicated protein purification targeted analysis required. Proteins fractionated qualitatively identified via LC-MS. A library built FASTA sequences yeast to analyze samples. fractionation successful at purifying isolating Two peaks sample, while one peak sample. LC/MS discovered presence flocculation proteins, particularly Flo1 Flo9. Understanding source these hazes can provide an opportunity for brewers mitigate against their formation by adjusting brewing management practices.

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ژورنال

عنوان ژورنال: Journal of The American Society of Brewing Chemists

سال: 2021

ISSN: ['1943-7854', '0361-0470']

DOI: https://doi.org/10.1080/03610470.2021.1937461